To prepare for this assignment, students should review and learn the material and lecture notes previously delivered in BTEC8002 about SDS-PAGE, qPCR, mammalian cell transfection, reverse transcription, production of a library and Illumina NGS. Completion of this assignment will: • Support students in learning the content delivered before the mid-semester break • Illustrate application of these methods and techniques The short question and answer section will assist you in reading the paper insightfully and will also assess your ability to apply your theoretical knowledge (from the lectures) to comprehend the research conducted in the manuscript. The last task is then to write a critique of the manuscript. Remember, a critique should always be constructive (improving the ability to present data or to write, suggest better suited/superior approaches, improve language and grammar, citations etc.). Critique of a manuscript can be positive or negative as long as the critique is demonstrated to be justifiable. Unless the paper is utter nonsense and poor research, for providing constructive critique it is advisable to first state what was done well, then critique the lower quality sections.