1. How is a DNA molecule prepared for observation with the electron microscope?
2. State two effects that a regulatory protein may have on gene expression.
3. State the theoretical basis of DNA foot-printing.
4. Outline the steps involved in DNA foot-printing.
5. State two uses of deletion analysis
6. What is a reporter gene?
7. State two criteria that are important in choice of a reporter gene.
8. Outline the steps involved in hybrid release translation (HRT)
9. Describe how a point mutation can be introduced at any point in a gene.
GENE TRANSFER TO PLANTS AN ANIMALS
1 Explain how a transgenic plant could be obtained from protoplasts
2 Explain how the biolistic technique can be used to obtain transgenic plants
3 Explain how transgenic mice could be produced by microinjection